Filamentous-haemagglutinin-like protein genes encoded on a plasmid of Moraxella bovis

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Tsutomu Kakuda1, Nopporn Sarataphan, Tetsuya Tanaka

  • 1Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University, Higashi 23, 35-1, Towada, Aomori 034-8628, Japan. kakuda@vmas.kitasato-u.ac.jp

Veterinary Microbiology
|August 2, 2006
PubMed

Insights

The Moraxella bovis plasmid pMBO-1 contains genes (flpA, flpB, fap) similar to Bordetella pertussis FHA, which are transcribed and present in diverse clinical isolates.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genomics

Background:

  • Moraxella bovis is an important pathogen in cattle.
  • Plasmids play a significant role in bacterial adaptation and virulence.
  • Understanding plasmid-borne genes in M. bovis can reveal mechanisms of pathogenesis.

Purpose of the Study:

  • To determine the complete nucleotide sequence of the plasmid pMBO-1 from Moraxella bovis strain Epp63.
  • To identify and characterize genes with potential roles in M. bovis virulence and adaptation.
  • To investigate the presence and transcription of identified genes in clinical isolates.

Main Methods:

  • Plasmid DNA isolation and sequencing.
  • Open reading frame (ORF) identification and analysis.
  • Reverse transcriptase PCR (RT-PCR) for gene transcription analysis.
  • Southern blot hybridization for gene distribution analysis.

Main Results:

  • The 44,215bp plasmid pMBO-1 was fully sequenced, revealing 30 ORFs.
  • Two large ORFs, flpA and flpB, encoding proteins homologous to Bordetella pertussis filamentous hemagglutinin (FHA), were identified.
  • A gene for an accessory protein (Fap), potentially involved in Flp secretion, was also found.
  • RT-PCR confirmed the transcription of flpA, flpB, and fap genes.
  • Southern blot analysis showed that flp and fap genes are present in geographically diverse M. bovis clinical isolates.

Conclusions:

  • The pMBO-1 plasmid harbors genes (flpA, flpB, fap) with potential implications for Moraxella bovis pathogenesis.
  • These genes are actively transcribed and conserved across different M. bovis clinical strains, suggesting functional importance.

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