Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Yeast ntr1/spp382 mediates prp43 function in postspliceosomes.

Kum-Loong Boon1, Tatsiana Auchynnikava, Gretchen Edwalds-Gilbert

  • 1Wellcome Trust Centre for Cell Biology, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, United Kingdom.

Molecular and Cellular Biology
|August 2, 2006
PubMed
Summary

The Ntr1 protein in yeast is crucial for efficient pre-messenger RNA (mRNA) splicing. It facilitates the removal of excised introns and the recycling of spliceosomal small nuclear RNAs (snRNAs), ensuring proper gene expression.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of an <i>In Vitro</i> Assay for Evaluation of Intracellular SPAAC Reactions Using Hoechst-DBCO.

Chemical & biomedical imaging·2026
Same author

Ribosomal RNA synthesis by RNA polymerase I is subject to premature termination of transcription.

eLife·2026
Same author

Biological evaluation of a glucose-based boron carrier as a potential agent for boron neutron capture therapy.

International journal of cancer·2025
Same author

Rapid cleavage of 6-[<sup>18</sup>F]fluoronicotinic acid prosthetic group governs BT12 glioblastoma xenograft uptake: implications for radiolabeling design of biomolecules.

EJNMMI radiopharmacy and chemistry·2025
Same author

Swt21p Is Required for Nam8p-U1 snRNP Association and Efficient Pre-mRNA Splicing in <i>Saccharomyces cerevisiae</i>.

International journal of molecular sciences·2025
Same author

Killer toxin K28 resistance in yeast relies on COG complex-mediated trafficking of the defence factor Ktd1.

Journal of cell science·2025

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • RNA Processing

Background:

  • The Ntr1 and Ntr2 proteins in Saccharomyces cerevisiae are known to interact with splicing factors.
  • Their specific roles in the pre-mRNA splicing pathway remain largely uncharacterized.

Purpose of the Study:

  • To elucidate the function of Ntr1 and Ntr2 proteins in the pre-mRNA splicing process.
  • To investigate the association of Ntr1 and Ntr2 with spliceosomal complexes and their impact on intron removal and snRNP recycling.

Main Methods:

  • Metabolic depletion of Ntr1 protein in yeast cells.
  • Analysis of splicing activity and accumulation of excised introns in depleted extracts.
  • Assessment of spliceosomal small nuclear RNA (snRNA) levels and their association with specific proteins (e.g., Prp8).

Related Experiment Videos

  • Investigation of Ntr1 interaction with the intron release factor Prp43.

    • Main Results:

    • Ntr1 and Ntr2 associate with postsplicing complexes containing excised introns and snRNAs (U2, U5, U6).
    • Ntr1 depletion leads to reduced splicing activity, accumulation of excised introns, and altered snRNP composition.
    • Ntr1 is required for the association of the intron release factor Prp43 with excised introns.

    Conclusions:

    • Ntr1 plays a critical role in the turnover of excised intron complexes and the recycling of spliceosomal snRNPs.
    • Ntr1 likely functions as a spliceosome receptor or RNA-targeting factor for Prp43, promoting intron release, potentially with assistance from Ntr2.