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Updated: Jul 6, 2026

In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS
Published on: February 15, 2016
Distinct dynamic behaviors of water molecules in hydrated pores
Martín Febles1, Natalia Pérez-Hernandez, Cirilo Pérez
1Instituto de Bioorganica, Universidad de La Laguna, CSIC, Ctra. Vieja de La Esperanza 2, 38206 La Laguna, Tenerife, Spain.
Abstract:
Water molecules confined inside narrow pores are of great importance in understanding the structure, stability, and function of water channels. Here we report that besides the H-bonding water that structures the pore, the permanent presence of a significant, fast-moving fraction of incompletely H-bonded water molecules inside the pore should control the free entry and exit of water. This is achieved by means of complementary DSC and solid-state NMR studies. We also present compelling evidence from X-ray diffraction data that the cluster formed by six water molecules in the most stable cage-like structure is sufficiently hydrophobic to be stably adsorbed in a nonpolar environment.
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