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Mapping the Arabidopsis genome.

B M Hauge¹, S Hanley, J Giraudat

¹Department of Genetics, Harvard Medical School, Boston, MA.

Symposia of the Society for Experimental Biology

|January 1, 1991

Summary

Researchers created a physical map of the Arabidopsis thaliana genome using cosmid clones and computer analysis. This map, combined with an RFLP map, simplifies gene cloning by connecting genetic loci to cloned genes.

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Area of Science:

Genomics
Plant Biology
Molecular Genetics

Background:

The Arabidopsis thaliana genome is a model system for plant research.
Assembling a complete physical map is crucial for understanding plant genomes.
Existing genetic maps require integration with physical maps for efficient gene cloning.

Purpose of the Study:

To construct a comprehensive physical map of the Arabidopsis thaliana genome.
To integrate the physical map with a Restriction Fragment Length Polymorphism (RFLP) map.
To develop a robust tool for simplifying gene cloning in Arabidopsis.

Main Methods:

Analysis of approximately 20,000 random cosmid clones with computer matching programs.
Assembly of cosmid clones into contigs representing 90-95% of the genome.
Construction of an RFLP map with 175 markers to bridge physical and genetic maps.

Main Results:

Successfully assembled ~750 contigs covering 90-95% of the Arabidopsis genome.
Developed an RFLP map providing 175 markers for integration.
Established contact points between the physical map and the classical genetic map.

Conclusions:

The combined RFLP and physical map serves as a valuable tool for gene cloning.
This integrated map facilitates the movement from a known genetic locus to the corresponding cloned gene.
The project significantly advances the ability to clone genes with unknown products in Arabidopsis.

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