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Visual Detection of Multiple Nucleic Acids in a Capillary Array
Published on: November 15, 2017
Rapid and high-throughput forensic short tandem repeat typing using a 96-lane microfabricated capillary array
Stephanie H I Yeung1, Susan A Greenspoon, Amy McGuckian
1UCSF/UCB Joint Graduate Group in Bioengineering, University of California, Berkeley, CA 94720, USA.
Journal of Forensic Sciences
|August 3, 2006
Summary
A new 96-channel microfabricated capillary array electrophoresis (muCAE) device enables rapid forensic short tandem repeat (STR) typing. This high-throughput system accurately analyzes DNA samples, including mixtures and low DNA amounts, paving the way for integrated microdevices.
Area of Science:
- Forensic Science
- Analytical Chemistry
- Biotechnology
Background:
- Traditional capillary electrophoresis is time-consuming for high-throughput forensic analysis.
- Development of integrated microdevices is crucial for efficient DNA profiling.
Purpose of the Study:
- To evaluate a 96-channel microfabricated capillary array electrophoresis (muCAE) device for forensic short tandem repeat (STR) typing.
- To assess the system's speed, resolution, and accuracy with established multiplex PCR systems.
Main Methods:
- Utilized a 96-channel muCAE device for STR analysis.
- Employed PowerPlex 16 and AmpFlSTR Profiler Plus multiplex PCR systems.
- Validated results against ABI Prism 310 and Hitachi FMBIO II systems.
Main Results:
- Achieved high-speed (<30 min) parallel sample separations with single-base resolution.
- Accurately typed 48 single-source samples, including minor alleles in mixture samples.
- Obtained full DNA profiles from as little as 0.17 ng of DNA, comparable to existing systems.
- Successfully analyzed 17 nonprobative forensic biological stain samples.
Conclusions:
- The 96-channel muCAE device demonstrates high performance for forensic STR typing.
- This technology offers a significant advancement towards fully integrated STR analysis microdevices.
- The system provides a viable high-throughput solution for forensic DNA analysis.
