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Related Experiment Videos

Temperature-induced cell detachment on immobilized pluronic surface.

Akon Higuchi1, Nobuo Aoki, Taro Yamamoto

  • 1Department of Materials and Life Science, Seikei University, 3-3-1 Kichijoji Kitamachi, Musashino, Tokyo 180-8633, Japan. higuchi@st.seikei.ac.jp

Journal of Biomedical Materials Research. Part A
|August 3, 2006
PubMed
Summary

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Pluronic F127-immobilized flasks create a bio-inert cell culture environment, enhancing hematopoietic stem cell marker expression. These flasks also allow for temperature-dependent cell detachment, improving cell recovery.

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Stem Cell Research

Background:

  • Conventional tissue culture flasks can influence cell behavior and marker expression.
  • Developing novel surfaces for cell culture is crucial for improving stem cell research and applications.
  • Pluronic copolymers offer tunable surface properties for biomaterial applications.

Purpose of the Study:

  • To immobilize Pluronic F68 and F127 copolymers onto tissue culture flasks.
  • To investigate the effect of Pluronic-immobilized surfaces on fibroblast and hematopoietic stem cell behavior.
  • To evaluate the potential of Pluronic-immobilized flasks for improved stem cell culture and recovery.

Main Methods:

  • Carbonyldiimidazole (CDI) activation of Pluronic F68 and F127 copolymers.

Related Experiment Videos

  • Immobilization of activated Pluronics onto poly-L-lysine-coated polystyrene tissue culture flasks.
  • Cell culture of L929 fibroblasts and umbilical cord blood-derived hematopoietic stem cells.
  • Analysis of cell morphology, spreading, and surface marker expression (CD34, CD133).

    • Main Results:

    • Pluronic F127-immobilized flasks created a bio-inert environment, resulting in spherical L929 fibroblast morphology with reduced spreading.
    • L929 cells could be detached from Pluronic-immobilized flasks via temperature reduction (4-15°C) due to Pluronic hydration/dehydration properties.
    • Hematopoietic stem cells cultured on Pluronic F127-immobilized flasks showed significantly higher expression of CD34 and CD133 markers compared to conventional flasks.

    Conclusions:

    • Pluronic-immobilized surfaces can modulate cell morphology and promote a bio-inert culture environment.
    • Temperature-controlled cell detachment from Pluronic-immobilized surfaces offers a gentle recovery method.
    • Pluronic F127-immobilized flasks significantly enhance the maintenance of hematopoietic stem cell surface marker expression, indicating improved stem cell culture quality.