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Effect of resveratrol on NF-kappaB activity in rat peritoneal macrophages
Zhen-Hua Ma1, Qing-Yong Ma, Lian-Cai Wang
1Division of General Surgery of First Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China. mazhenhua2005@163.com
Abstract:
This study was to investigate the inhibitive effect of resveratrol (RESV) on nuclear factor kappa B (NF-kappaB) expression and activity induced by lipopolysaccharide (LPS) in rat peritoneal macrophages (PMA). Male Sprague-Dawley (SD) rats were randomly divided into 7 groups, including control group, LPS group and RESV I-V group. In the LPS group, PMA were incubated in DMEM containing LPS (10 microg/ml), whereas in control group, PMA were incubated in DMEM only. In the RESV I-V groups, PMA were incubated in DMEM containing LPS (10 microg/ml) and different concentrations of RESV. After 24 hours of incubation, NF-kappaB activity in PMA, and the levels of tumor necrosis factor alpha (TNF-alpha), interleukin-1 (IL-1) and nitric oxide (NO) in the culture medium were measured. In the concentrations of 1.25-5 microg/ml, RESV had a dose- dependent inhibitive effect on NF-kappaB activity in PMA as well as the expressions of TNF-alpha, IL-1 and NO in the culture medium contrasted with the LPS group. There was no significant difference in the levels of these pro-inflammatory factors between the groups of 5 microg/ml and 10 microg/ml RESV. In conclusion, RESV has the potential for the future application of preventing inflammatory diseases involving PMA.
Insights
Resveratrol (RESV) effectively inhibits nuclear factor kappa B (NF-kappaB) activation and pro-inflammatory markers like TNF-alpha and IL-1 in rat macrophages. This suggests RESV
Area of Science:
- Immunology
- Pharmacology
- Cell Biology
Background:
- Lipopolysaccharide (LPS) is a potent inducer of inflammation in macrophages.
- Nuclear factor kappa B (NF-kappaB) signaling is a key pathway in inflammatory responses.
- Resveratrol (RESV) is a natural polyphenol with known anti-inflammatory properties.
Purpose of the Study:
- To investigate the inhibitory effects of resveratrol (RESV) on LPS-induced NF-kappaB activation.
- To evaluate the impact of RESV on pro-inflammatory cytokine and nitric oxide production in macrophages.
- To determine the dose-dependent efficacy of RESV in modulating inflammatory pathways.
Main Methods:
- Primary rat peritoneal macrophages (PMA) were isolated from Sprague-Dawley rats.
- Macrophages were stimulated with LPS (10 microg/ml) and treated with varying concentrations of RESV (1.25-10 microg/ml).
- NF-kappaB activity, TNF-alpha, IL-1, and nitric oxide (NO) levels were measured after 24 hours of incubation.
Main Results:
- RESV demonstrated a dose-dependent inhibition of NF-kappaB activity in LPS-stimulated PMA.
- RESV significantly reduced the expression of TNF-alpha, IL-1, and NO in the culture medium.
- Inhibitory effects were observed at RESV concentrations ranging from 1.25 to 5 microg/ml, with no significant difference between 5 and 10 microg/ml.
Conclusions:
- Resveratrol effectively suppresses LPS-induced NF-kappaB activation and pro-inflammatory mediator release in rat macrophages.
- RESV shows potential as a therapeutic agent for preventing inflammatory diseases.
- These findings highlight the anti-inflammatory capacity of RESV in a cellular model.
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