Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Head module control of mediator interactions.

Yuichiro Takagi1, Guillermo Calero, Hirofumi Komori

  • 1Department of Structural Biology, Stanford University School of Medicine, Stanford, California 94305, USA.

Molecular Cell
|August 4, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Workflow for Visualizing Protein Conformational Dynamics from Cryo-EM Maps Using Human Asparagine Synthetase.

Journal of visualized experiments : JoVE·2026
Same author

Rapidly evolving aphid gall effector proteins exhibit saposin-like folds.

bioRxiv : the preprint server for biology·2026
Same author

Impact of running average value of non-fasting serum triglyceride for secondary prevention of coronary artery disease.

Journal of cardiology·2026
Same author

Mining antibody functionality via AI-guided structural landscape profiling.

Nature communications·2026
Same author

Conformational Remodeling Underlies Activity Loss in Disease-Linked Asparagine Synthetase Variant.

bioRxiv : the preprint server for biology·2026
Same author

Mechanistic insights into histone recognition and H3K14 acetylation by the NuA3 histone acetyltransferase complex.

Nature communications·2025
Same journal

Biomolecular condensates for proteostasis and potential therapeutic applications.

Molecular cell·2026
Same journal

A negative regulator of mitochondrial complex I assembly adapts respiration to cellular energy demand.

Molecular cell·2026
Same journal

Large-scale tethered screen of RNA-binding proteins reveals novel regulators of poly(A) site selection.

Molecular cell·2026
Same journal

Longitudinal monitoring of cytoplasmic RBP-RNA interactions and transcriptome in living cells by engineered protein nanocages.

Molecular cell·2026
Same journal

Structures of the PI3Kα/KRas complex on lipid bilayers reveal molecular mechanisms of PI3Kα activation.

Molecular cell·2026
Same journal

Oligomer disassembly activates an HEPN-containing bacterial defense system.

Molecular cell·2026
See all related articles

The yeast Mediator head module, a key protein complex, was purified and characterized. This module is crucial for RNA polymerase II transcription initiation and promoter interactions.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Yeast Genetics

Background:

  • The Mediator complex is essential for regulating gene transcription by RNA polymerase II.
  • Specific subunits and their functions within the Mediator complex are not fully understood.

Purpose of the Study:

  • To express, purify, and characterize the yeast Mediator head module.
  • To investigate the role of the Mediator head module in transcription initiation and its interactions with RNA polymerase II and DNA templates.

Main Methods:

  • Recombinant baculovirus expression system for high-level protein production.
  • Purification of a seven-subunit Mediator complex (223 kDa).
  • Electron microscopy and single-particle analysis for structural identification.
  • In vitro transcription assays using recombinant Mediator head module.

Related Experiment Videos

  • Analysis of Mediator complex stability in vivo using temperature-sensitive mutants.

    • Main Results:

    • A homogeneous seven-subunit Mediator head module was successfully purified.
    • The recombinant head module rescued transcription initiation in vitro.
    • The head module specifically interacts with the RNA polymerase II-TFIIF complex, and this interaction is disrupted by DNA and RNA.
    • In vivo disruption of the head module caused the release of other Mediator subunits from promoters.

    Conclusions:

    • The yeast Mediator head module is a distinct functional entity.
    • The head module plays a critical role in mediating interactions between Mediator, RNA polymerase II, and promoter DNA.
    • Mediator subunit release during transcription initiation is controlled by the head module.