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Published on: June 30, 2016
Two GacA-dependent small RNAs modulate the quorum-sensing response in Pseudomonas aeruginosa
Elisabeth Kay1, Bérénice Humair, Valérie Dénervaud
1Département de Microbiologie Fondamentale, Université de Lausanne, Building Biophore, CH-1015 Lausanne, Switzerland.
Abstract:
In Pseudomonas aeruginosa, the GacS/GacA two-component system positively controls the quorum-sensing machinery and the expression of extracellular products via two small regulatory RNAs, RsmY and RsmZ. An rsmY rsmZ double mutant and a gacA mutant were similarly impaired in the synthesis of the quorum-sensing signal N-butanoyl-homoserine lactone, the disulfide bond-forming enzyme DsbA, and the exoproducts hydrogen cyanide, pyocyanin, elastase, chitinase (ChiC), and chitin-binding protein (CbpD). Both mutants showed increased swarming ability, azurin release, and early biofilm development.
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