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Related Experiment Videos

Protooncogene expression in normal and psoriatic skin.

J T Elder1, A Tavakkol, S B Klein

  • 1Department of Dermatology, University of Michigan, Ann Arbor.

The Journal of Investigative Dermatology
|January 1, 1990
PubMed
Summary

Protooncogene expression in psoriasis was studied. While c-myc levels were elevated in psoriatic skin, normalization revealed no significant increase, suggesting protooncogenes are not central to psoriasis pathogenesis.

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Area of Science:

  • Dermatology
  • Molecular Biology
  • Oncology

Background:

  • Psoriasis is a chronic inflammatory skin condition characterized by epidermal hyperplasia.
  • Protooncogenes play a role in cell growth and differentiation, and their dysregulation is implicated in various cancers.

Purpose of the Study:

  • To compare the expression levels of specific protooncogenes (c-myc, c-fos, c-jun, c-erbB, c-Ha-ras) in normal skin versus psoriatic plaques.
  • To investigate the role of these protooncogenes in the pathogenesis of psoriatic epidermal hyperplasia.

Main Methods:

  • Northern blot analysis of total RNA extracted from normal skin and psoriatic plaque keratome biopsies.
  • Densitometric analysis to quantify transcript levels.
  • Normalization of protooncogene mRNA levels to housekeeping genes (cyclophilin, lipocortin II).

Related Experiment Videos

  • Organ culture of tissue biopsies to assess gene induction after injury.

    • Main Results:

    • Initial analysis showed 1.5- to 2.5-fold elevations of c-myc transcripts in psoriatic lesions compared to normal skin.
    • After normalization to cyclophilin or lipocortin II, c-myc, c-jun, c-erbB, c-fos, and c-Ha-ras transcript levels were not significantly increased in psoriatic epidermis.
    • Transforming growth factor-alpha (TGF-alpha) transcripts were significantly elevated (10- to 20-fold) in psoriatic lesions.
    • C-myc, c-fos, and c-jun transcripts showed significant induction after organ culture, indicating responsiveness to tissue injury.

    Conclusions:

    • Overexpression of the studied protooncogenes is not a primary factor in the epidermal hyperplasia seen in psoriasis.
    • TGF-alpha may play a more significant role in the pathogenesis of psoriatic skin lesions.
    • Protooncogene expression can be rapidly induced by tissue injury, independent of the psoriatic condition.