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Sequestration of eosinophil major basic protein in human mast cells
J H Butterfield1, D Weiler, E A Peterson
1Department of Dermatology, Mayo Clinic, Rochester, Minnesota.
Summary
Human mast cells, particularly in nasal polyps and skin lesions, can contain eosinophil major basic protein (MBP). This suggests mast cells may internalize toxic eosinophil proteins through endocytosis.
Area of Science:
- Immunology
- Cell Biology
- Pathology
Background:
- Previous research indicated lung and skin mast cells lack eosinophil major basic protein (MBP).
- A new human mast cell line showed immunofluorescence for MBP.
- Mast cell heterogeneity is increasingly recognized.
Purpose of the Study:
- To investigate the presence of MBP in human mast cells across various tissues.
- To determine if mast cells can acquire eosinophil proteins.
Main Methods:
- Mast cell identification using specific stains (avidin, esterases).
- Immunofluorescence and immunoelectron microscopy for MBP localization.
- In vivo experiment involving MBP injection into normal skin.
Main Results:
- MBP was found in mast cells from nasal polyps, ileal tissue, and cutaneous mastocytosis specimens.
- Normal skin mast cells did not contain MBP.
- Mast cells rapidly acquired MBP after injection, indicating endocytosis.
Conclusions:
- Human mast cells in specific tissues can contain and sequester eosinophil MBP.
- Endocytosis appears to be the mechanism by which mast cells acquire MBP.
- This finding has implications for understanding mast cell heterogeneity and function in inflammatory conditions.