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Updated: Aug 6, 2026
Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis
Published on: May 9, 2025
[Effect of interferon-gamma on bleomycin induced pulmonary fibrosis in rats]
De-ping Zhang1, Hui Qiu, Yi Zhuang
1Department of Respiratory Medicine, Drum Tower Hospital, Nanjing University Medical College, Nanjing 210008, Jiangsu, China.
Objective:
To observe the effects of interferon-gamma (IFN-gamma) on lung injury induced by bleomycin and its mechanism.
Methods:
Sixty-six SD rats were randomly divided into three group: the normal control group (group N, n=24), the bleomycin group (group B, n=24) and IFN-gamma treatment group (group I, n=18). The degree of lung injury and the concentrations of tumor necrosis factor-alpha (TNF-alpha) and IFN-gamma in bronchoalveolar lavage fluid (BALF) and serum were measured.
Results:
The degree of lung injury in group I was severer than group B, but with no significant difference. The concentration of TNF-alpha in BALF on 17, 21, 28 days and in serum on 21 and 28 days were significantly increased after IFN-gamma administration compared with group N and group B (all P<0.05). The concentration of IFN-gamma in BALF and serum increased on 21 and 28 days in group I (all P<0.05).
Conclusion:
IFN-gamma might aggravate lung injury induced by bleomycin, and it might attribute to an increase in TNF-alpha.
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