Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA sequence-directed assembly of two peptide bioconjugates.

M Thompson1

  • 1Department of Chemistry, Michigan Technological University, Houghton, MI 49931, USA. mthomps@mtu.edu

Bioorganic Chemistry
|August 5, 2006
PubMed
Summary

Peptide bioconjugates, combining DNA-binding proteins and fluorescent dyes, enable sensitive detection of DNA bending. These probes offer insights into transcription initiation by analyzing protein-DNA interactions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Co-transmission of conduct problems with attention-deficit/hyperactivity disorder: familial evidence for a distinct disorder.

Journal of neural transmission (Vienna, Austria : 1996)·2008
Same author

A high-density SNP linkage scan with 142 combined subtype ADHD sib pairs identifies linkage regions on chromosomes 9 and 16.

Molecular psychiatry·2008
Same author

Inhibitory deficits in attention-deficit/hyperactivity disorder are independent of basic processing efficiency and IQ.

Journal of neural transmission (Vienna, Austria : 1996)·2007
Same author

Endovascular management of chronic type B dissecting aortic aneurysm utilizing aortic and renal stents.

Cardiovascular and interventional radiology·2007
Same author

Lemierre's syndrome complicating pregnancy.

Infectious diseases in obstetrics and gynecology·2007
Same author

The use of an extra-corporeal graft to maintain cerebral perfusion during thoracic endovascular aneurysm repair.

European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·2007

Area of Science:

  • Bioconjugation Chemistry
  • Molecular Biology
  • Biophysics

Background:

  • Peptide bioconjugates integrate DNA-binding protein recognition with DNA-sensitive fluorescent dye properties.
  • Understanding protein-DNA interactions is crucial for deciphering gene regulation, particularly transcription initiation.

Purpose of the Study:

  • To investigate the DNA template-directed assembly of two bioconjugate probes.
  • To assess the sensitivity of these probes for detecting DNA structural changes and protein-induced bending.

Main Methods:

  • Utilized steady-state fluorescence resonance energy transfer (FRET) and time-resolved single photon counting.
  • Determined the Förster critical distance for the oxazole yellow and thiazole orange donor-acceptor pair.
  • Analyzed energy transfer efficiency based on intervening base pairs between DNA binding sites.

Related Experiment Videos

Main Results:

  • Established a Förster critical distance of approximately 26 Å for the specified dye pair.
  • Demonstrated that energy transfer efficiency correlates with the number of base pairs between DNA binding sites.
  • Showcased probe sensitivity in detecting sequence-dependent DNA curvature and polypeptide-induced DNA bending.

Conclusions:

  • Peptide bioconjugate probes are effective tools for studying DNA structure and protein interactions.
  • These molecular probes can provide valuable spatial information about protein complexes at promoter sites.
  • The findings contribute to understanding early events in transcription initiation.