Related Experiment Video
Updated: Aug 6, 2026

LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics
Published on: April 9, 2017
EPR spectroscopy and theoretical study of gamma-irradiated asparagine and aspartic acid in solid state
Grazyna Strzelczak1, Jacqueline Bergès, Chantal Houée-Levin
1Institute of Nuclear Chemistry and Technology, 03-195 Warsaw, Poland.
Abstract:
Aspartic acid (Asp) and asparagine (Asn) are vulnerable amino acids. One-electron addition or withdrawal reactions initiate many deleterious processes involving these amino acids. To study these redox processes we have irradiated by gamma-rays asparagine or aspartic acid in the solid state. The nature of the resulting free radicals was determined by electron paramagnetic resonance (EPR) and by calculations using DFT methods in various environments. Reactions initiated by electron transfer are different for both amino acids: Asn anion loses hydrogen atom whereas the cation undergoes decarboxylation. Conversely, Asp cation loses hydrogen atom from amine group, which triggers decarboxylation.
More Related Videos
10:03Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
Published on: June 27, 2014
08:48High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
Published on: April 28, 2022
Related Concept Videos
Atomic Emission Spectroscopy: Lab
IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations
Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals
NMR Spectroscopy Of Amines
Mass Spectrometry of Amines
Atomic Emission Spectroscopy: Overview