Comparative growth of Porphyromonas gingivalis strains in a defined basal medium

Porphyromonas gingivalis is an asaccharolytic bacterium whose metabolism is dependent on the uptake of small peptides and amino acids. The aim of this work was to study the growth of P. gingivalis in a defined basal medium (DBM) supplemented with various sources of proteins. The strain 49417 as well as other virulent isolates could grow in DBM containing 1% bovine serum albumin (BSA). Cells cultivated under this condition showed a slightly modified protein profile, and expressed hemagglutinating as well as proteolytic activities. Other natural proteins under investigation could not support the growth in the DBM. On the other hand, the strain 33277 as well as other avirulent strains of P. gingivalis could not use BSA as a substrate. The ability of P. gingivalis to grow in DBM-BSA is not entirely dependent on its ability to degrade the protein substrate as strain 33277 was able to extensively hydrolyse the molecule. Differences in either metabolic enzymes or peptide transport mechanisms may explain the distinctive behavior between virulent and avirulent strains. Data from this work suggest a relationship between nutritional requirements and virulence of P. gingivalis in an animal model. The DBM-BSA may represent a more appropriate medium for studies on the physiology of P. gingivalis.