Jove
Visualize
Contact Us

Related Experiment Videos

Binary toxin producing Clostridium difficile strains.

M Rupnik1, M Grabnar, B Geric

  • 1Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, Ljubljana SLO-1000, Slovenia. mrupnik1@gwdg.de

Anaerobe
|August 5, 2006
PubMed
Summary

Clostridium difficile produces binary toxin (CDT), an actin-specific toxin composed of CDTa and CDTb components. Sequencing identified CDT genes in positive strains and truncated genes in negative strains, impacting virulence factor understanding.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Room-temperature memristive switching between charge density wave states.

Nature communications·2026
Same author

Effects of Limosilactobacillus reuteri DSM 17938 in neonates exposed to antibiotics: a randomised controlled trial.

Beneficial microbes·2024
Same author

Zoonotic Transfer of Clostridium difficile Harboring Antimicrobial Resistance between Farm Animals and Humans.

Journal of clinical microbiology·2017
Same author

Molecular characteristics of Clostridium difficile isolates from human and animals in the North Eastern region of India.

Molecular and cellular probes·2016
Same author

Isolation and characterization of Clostridium difficile from pet dogs in Assam, India.

Anaerobe·2015
Same author

Survey of community-associated-methicillin-Resistant Staphylococcus aureus in Slovenia: identification of community-associated and livestock-associated clones.

International journal of medical microbiology : IJMM·2015
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Microbiology
  • Molecular Biology
  • Toxicology

Background:

  • Clostridium difficile produces toxins TcdA, TcdB, and CDT.
  • TcdA and TcdB are glucosyltransferases targeting small GTPases.
  • CDT is an ADP-ribosylating binary toxin targeting actin, comprising CDTa and CDTb components.

Purpose of the Study:

  • To sequence the cdtA and cdtB genes in CDT-positive and CDT-negative Clostridium difficile strains.
  • To investigate the prevalence and genetic basis of binary toxin production in C. difficile.
  • To discuss the role of CDT as a potential virulence factor.

Main Methods:

  • Gene sequencing of cdtA and cdtB in C. difficile strains CD 196, 8864, VPI 10463, and 630.
  • Analysis of gene sequences to identify functional and truncated forms of binary toxin genes.

Related Experiment Videos

Main Results:

  • The cdtA and cdtB genes were successfully sequenced in two CDT-positive C. difficile strains.
  • Two CDT-negative strains exhibited truncated forms of the binary toxin genes.
  • Prevalence of binary toxin producing strains estimated between 1.6% and 5.5%.

Conclusions:

  • The genetic basis for CDT production and its absence in certain strains has been elucidated.
  • Binary toxin (CDT) represents an additional virulence factor in Clostridium difficile.
  • Further research is warranted to fully understand the clinical significance of CDT.