Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hypoxia-inducible factor-1 (HIF-1).

Qingdong Ke1, Max Costa

  • 1Nelson Institute of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, NY 10987, USA.

Molecular Pharmacology
|August 5, 2006
PubMed
Summary

Cells adapt to low oxygen (hypoxia) by activating hypoxia-inducible factor-1 (HIF-1). This transcription factor regulates genes involved in survival and proliferation, and targeting it offers a potential cancer therapy approach.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Elevated SNHG1 promotes invasion and migration of Cd(II)-transformed cells through Sox2, Rac1, and Slug.

Toxicology and applied pharmacology·2025
Same author

Isorhapontigenin Inhibits Cell Growth, Angiogenesis, Migration, and Invasion of Non-Small-Cell Lung Cancer Cells Through NEDD9 Signaling.

International journal of molecular sciences·2025
Same author

Skyrocketing pollution: assessing the environmental fate of July 4th fireworks in New York City.

Journal of exposure science & environmental epidemiology·2024
Same author

Hexavalent chromium exposure activates the non-canonical nuclear factor kappa B pathway to promote immune checkpoint protein programmed death-ligand 1 expression and lung carcinogenesis.

Cancer letters·2024
Same author

Transcriptome Analysis Reveals Anti-Cancer Effects of Isorhapontigenin (ISO) on Highly Invasive Human T24 Bladder Cancer Cells.

International journal of molecular sciences·2024
Same author

Stabilization of MOF (KAT8) by USP10 promotes esophageal squamous cell carcinoma proliferation and metastasis through epigenetic activation of ANXA2/Wnt signaling.

Oncogene·2024

Area of Science:

  • Cellular and Molecular Biology
  • Physiology
  • Oncology

Background:

  • Cellular adaptation to low oxygen tension (hypoxia) involves transcriptional induction of genes critical for angiogenesis, metabolism, and survival.
  • Hypoxia-inducible factor-1 (HIF-1) is the master transcriptional regulator of the hypoxic response.
  • HIF-1 comprises a stable HIF-1beta subunit and an oxygen-sensitive HIF-1alpha subunit (and paralogs).

Purpose of the Study:

  • To elucidate the regulatory mechanisms of HIF-1alpha stability and activity.
  • To understand the role of HIF-1 in cellular adaptation to hypoxia.
  • To explore the therapeutic potential of targeting HIF-1 in cancer.

Main Methods:

  • Investigated post-translational modifications of HIF-1alpha, including hydroxylation, ubiquitination, acetylation, and phosphorylation.
  • Examined the interaction of HIF-1alpha with the pVHL E3 ligase complex under normoxia.
  • Analyzed HIF-1alpha stabilization and coactivator interaction under hypoxia.

Main Results:

  • HIF-1alpha stability is tightly regulated by proline hydroxylation and lysine acetylation within its oxygen-dependent degradation domain (ODDD).
  • Under normoxia, these modifications target HIF-1alpha for degradation via the ubiquitin-proteasome pathway.
  • Under hypoxia, HIF-1alpha accumulates, interacts with coactivators like p300, and drives target gene expression.

Conclusions:

  • HIF-1alpha post-translational modifications are crucial for oxygen sensing and regulating gene expression.
  • The HIF-1 pathway is frequently overexpressed in cancers.
  • Targeting HIF-1 represents a promising therapeutic strategy for cancer treatment.

Related Experiment Videos