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Updated: May 2, 2026

Ex Vivo Imaging of Postnatal Cerebellar Granule Cell Migration Using Confocal Macroscopy
Published on: May 12, 2015
PACAP and ceramides exert opposite effects on migration, neurite outgrowth, and cytoskeleton remodeling
Anthony Falluel-Morel1, David Vaudry, Nicolas Aubert
1INSERM U413, IFRMP 23, University of Rouen, 76821 Mont-Saint-Aignan, France.
Abstract:
During brain development, cells that fail to reach their final destination or to establish proper connections are eliminated. It has been shown that the proinflammatory cytokine second messenger ceramides and the neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP) play pivotal roles in the histogenesis of the cerebellum. However, little is known regarding the effects of these two factors on cerebellar granule cell migration. We have found that PACAP prevents the effects of C2-ceramide on granule cell motility and neurite outgrowth. These actions are attributable to opposite effects on actin distribution, tubulin polymerization, and Tau phosphorylation. These data suggest that PACAP and factors inducing ceramide production may control granule cell migration during cerebellar development.
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