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CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
Published on: June 25, 2017
Identification of repressor element 1 in secretin/PACAP/VIP genes
Leo T O Lee1, Vien H Y Lee, Paula Y Yuan
1Department of Zoology, The University of Hong Kong, Pokfulam Road, Hong Kong, PRC.
Abstract:
Repressor element 1 (RE-1) is a negative, cis-acting regulatory element that interacts with the transcription factor RE-1-silencing transcription factor (REST). REST represses gene expression by two repressor domains that recruit other factors including mSin3 and CoREST. RE-1 has been identified in an increasing number of neuronal-specific genes, and recently, functional REST sites have also been discovered in VIP and PACAP genes. In the present article, we demonstrated for the first time that RE-1 sites are present in the 5' flanking regions of several secretin/PACAP/VIP genes by in silico analysis. This observation suggests that RE-1/REST is a common negative regulatory pathway of this peptide family.
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