Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

VIP protects Th2 cells by downregulating granzyme B expression.

Vikas Sharma1, Mario Delgado, Doina Ganea

  • 1Department of Biological Sciences, Rutgers University, Newark, New Jersey 07102, USA.

Annals of the New York Academy of Sciences
|August 5, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cortistatin as a Novel Multimodal Therapy for the Treatment of Parkinson's Disease.

International journal of molecular sciences·2024
Same author

A potential patient stratification biomarker for Parkinson´s disease based on LRRK2 kinase-mediated centrosomal alterations in peripheral blood-derived cells.

NPJ Parkinson's disease·2024
Same author

Novel Therapeutic Opportunities for Neurodegenerative Diseases with Mesenchymal Stem Cells: The Focus on Modulating the Blood-Brain Barrier.

International journal of molecular sciences·2023
Same author

Therapeutic Effect of a Latent Form of Cortistatin in Experimental Inflammatory and Fibrotic Disorders.

Pharmaceutics·2022
Same author

Editorial: The innate immune system in rheumatoid arthritis.

Frontiers in immunology·2022
Same author

Efficacy of Vafidemstat in Experimental Autoimmune Encephalomyelitis Highlights the KDM1A/RCOR1/HDAC Epigenetic Axis in Multiple Sclerosis.

Pharmaceutics·2022

Vasoactive intestinal peptide (VIP) protects T helper 2 (Th2) cells from death by preventing granzyme B (GrB) upregulation. This mechanism is key for Th2 cell survival and immune response regulation.

Area of Science:

  • Immunology
  • Cellular Biology
  • Molecular Medicine

Background:

  • T helper (Th) cell differentiation into Th1 and Th2 effectors is crucial for adaptive immunity.
  • Vasoactive intestinal peptide (VIP) is known to promote Th2 differentiation and survival.
  • The precise mechanisms underlying VIP's protective effects on Th2 cells require further elucidation.

Purpose of the Study:

  • To investigate the molecular mechanisms by which VIP enhances Th2 cell survival.
  • To identify key molecules involved in VIP-mediated protection of Th2 cells.
  • To explore the role of granzyme B (GrB) in T helper cell apoptosis.

Main Methods:

  • Microarray analysis to assess gene expression changes in Th1 and Th2 cells.
  • Protein expression analysis.

Related Experiment Videos

  • Investigation of T helper cell apoptosis pathways.

    • Main Results:

    • VIP treatment prevented the upregulation of granzyme B (GrB) specifically in Th2 effector cells, while Th1 cells showed no such effect.
    • Granzyme B (GrB) expression was identified as a significant factor in activation-induced apoptosis of T helper cells.
    • Th2 cells exhibited enhanced responsiveness to VIP, likely due to higher VIP receptor expression and distinct signaling pathways.

    Conclusions:

    • Granzyme B (GrB) is a newly identified critical mediator in the activation-induced cell death of Th1/Th2 cells.
    • VIP confers a protective effect on Th2 cell survival by inhibiting GrB upregulation.
    • Understanding these mechanisms provides insights into immune regulation and potential therapeutic targets.