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Related Experiment Videos

Involucrin-like proteins in non-primates.

J Kubilus1, S B Phillips, M A Goldaber

  • 1Department of Dermatology, Massachusetts General Hospital, Boston 02114.

The Journal of Investigative Dermatology
|February 1, 1990
PubMed
Summary

Researchers investigated human involucrin (a protein) in various species using antibodies. Involucrin-like proteins were detected in monkeys, cows, dogs, sheep, guinea pigs, rats, and whales, suggesting a broader evolutionary presence.

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Area of Science:

  • Immunology
  • Comparative Biology
  • Biochemistry

Background:

  • Human involucrin is a key protein in epidermal differentiation.
  • Its presence and function in other species remain largely unexplored.

Purpose of the Study:

  • To investigate the presence of involucrin or involucrin-like proteins in various animal species.
  • To characterize the cross-reactivity of antibodies against human involucrin in non-human species.

Main Methods:

  • Utilized monoclonal and polyclonal antibodies specific to human involucrin.
  • Performed Western blot analysis on protein extracts from various species.
  • Purified immunoreactive proteins from cow and dog for further characterization.

Main Results:

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  • Antibodies detected homologous proteins in monkeys, cows, and dogs.
  • One antibody cross-reacted with proteins in sheep, guinea pigs, rats, and finback whales.
  • Purified proteins from cows and dogs showed cross-reactivity with human involucrin antibodies, with dog protein binding inhibited by human involucrin.

Conclusions:

  • Involucrin-like proteins are present across a wider range of mammalian and even some non-mammalian species than previously known.
  • These findings suggest an ancient evolutionary origin and conserved function for involucrin-like proteins.