Related Experiment Video
Updated: Aug 6, 2026

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
Published on: June 25, 2017
Insulin resistance does not influence gene expression in skeletal muscle
Lisa L Nguyen1, Adamandia D Kriketos, Dale P Hancock
1School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW, Australia. L.Nguyen@mmb.usyd.edu.au
Abstract:
Insulin resistance is commonly observed in patients prior to the development of type 2 diabetes and may predict the onset of the disease. We tested the hypothesis that impairment in insulin stimulated glucose-disposal in insulin resistant patients would be reflected in the gene expression profile of skeletal muscle. We performed gene expression profiling on skeletal muscle of insulin resistant and insulin sensitive subjects using microarrays. Microarray analysis of 19,000 genes in skeletal muscle did not display a significant difference between insulin resistant and insulin sensitive muscle. This was confirmed with real-time PCR. Our results suggest that insulin resistance is not reflected by changes in the gene expression profile in skeletal muscle.
More Related Videos
Related Concept Videos
Insulin: The Receptor and Signaling Pathways
Cell Specific Gene Expression
Type II Diabetes II: Pathophysiology
Genomic Imprinting and Inheritance
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
Type II Diabetes I: Introduction
Oral Hypoglycemic Agents: Biguanides and Glitazones

