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Updated: Aug 6, 2026
Harvesting Murine Alveolar Macrophages and Evaluating Cellular Activation Induced by Polyanhydride Nanoparticles
Published on: June 8, 2012
[The effect of ultra high molecular weight polyethylene particles on macrophage]
Yuan-qing Mao1, Zhen-an Zhu, Ting-ting Tang
1Department of Orthopedic Surgery, the Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200011, China. maoyuanqing@gmail.com
Objective:
To study the effect of ultra high molecular weight polyethylene (UHMWPE) particles on macrophages and evaluate the expression of NFAT2, a key transcriptional factor for osteoclast differentiation.
Methods:
From November 2004 to February 2005, macrophages were co-cultured with UHMWPE particles. When observed at different times, the proliferation activity of macrophages was analyzed by MTT and the expression of calcineurin (CaN) and NFAT2 by immunohistochemical and RT-PCR method respectively.
Results:
The macrophages phagocytosed UHMWPE particles in an early time, the expression of CaN and NFAT2 was increased, while the proliferation activity was not enhanced.
Conclusions:
UHMWPE particles can stimulate macrophages to phagocytose significantly, and enhance the expression of the transcriptional factor NFAT2.
