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Updated: Aug 6, 2026

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
12:23

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

Emerging applications for phospho-proteomics in cancer molecular therapeutics

Michael F Moran1, Jiefei Tong, Paul Taylor

  • 1Cancer Program, Hospital For Sick Children, and McLaughlin Centre for Molecular Medicine, and Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada. m.moran@utoronto.ca

Biochimica Et Biophysica Acta
|August 8, 2006
PubMed

Insights

Identifying patients for targeted cancer therapies is challenging. Phospho-proteomics offers potential biomarkers to monitor drug effects on kinase pathways, but requires overcoming technical and bioinformatics hurdles.

Area of Science:

  • Cellular biology
  • Biochemistry
  • Oncology

Background:

  • Protein phosphorylation regulates normal and cancer cell functions.
  • Targeted cancer therapies focus on protein kinase pathways.
  • Patient selection for kinase-targeted drugs remains a significant challenge.

Purpose of the Study:

  • To explore the potential of phospho-proteomics as a biomarker for monitoring drug-targeted kinase pathways.
  • To address the need for biomarkers in personalized cancer therapy.

Main Methods:

  • Phospho-proteomics involves enriching phosphorylated proteins from tissues.
  • Mass spectrometry (MS) is used for identifying and quantifying phosphorylation sites.
  • This approach aims to provide pharmacodynamic readouts of drug responses.

Main Results:

  • Phospho-proteomics has the potential to offer pharmacodynamic readouts.
  • It can provide insights into disease states and cellular drug responses in tumors.

Conclusions:

  • Phospho-proteomics shows promise for monitoring targeted cancer therapy effectiveness.
  • Technical and bioinformatics challenges must be overcome for clinical application.

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