Related Experiment Video
Updated: Aug 6, 2026
Electroporation of Plasmid DNA into Mouse Skeletal Muscle
Published on: April 6, 2022
High efficiency gene delivery into laryngeal muscle with bidirectional electroporation
Koichiro Saito1, Mohamed Lehar, Zhao Bo Li
1Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, 601 North Caroline Street, Baltimore, MD 21287, USA.
Objective:
The impact of polarity change on the efficiency of in vivo electroporative (EP) gene transfection was assessed in rat laryngeal muscle.
Study Design And Setting:
High (HV) and low field voltage (LV) were combined with polarity changes to determine transfection in 5 different conditions: 1) without EP (EP[-]), 2) HV+LV (HL), 3) HV+LV followed by HV+LV with no change in polarity (HLHL unidirectional), 4) HV+LV followed by HV+LV with opposite polarity (HLHL bidirectional), 5) HV+LV followed by LV with opposite polarity (HLL bidirectional).
Results:
HLL bidirectional sequence showed the best result with less interindividual variability and extended expression period. With the exception of repeated high voltage sequences, EP parameters were not likely to induce cell injury or inflammation.
Conclusion:
HLL bidirectional electroporative gene delivery produces high transfection rates with limited tissue trauma.
Significance:
Bidirectional EP provides a safe and highly efficient method for therapeutic gene delivery into skeletal muscle.
