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Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model
Published on: September 29, 2011
Accumulation of oxidative damage during replicative aging of the yeast Saccharomyces cerevisiae
Agnieszka Grzelak1, Ewa Macierzyńska, Grzegorz Bartosz
1Department of Molecular Biophysics, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland. agrzelak@biol.uni.lodz.pl
Abstract:
Comparison of senescent yeast obtained by the "baby machine" technique with 2-day-old stationary phase cells revealed decreased activities of glutathione reductase, glutathione S-transferase, glutathione peroxidase and alcohol dehydrogenase, reduction of total antioxidant capacity, protein glycation and accumulation of products of oxidative damage: protein carbonyls and DNA damage assessed by augmented content of 8-oxoguanine and increased tail momentum of cellular DNA in the comet assay. These results are consistent with a role for oxidative damage during replicative senescence of Saccharomyces cerevisiae.
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