Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

New Staphylococcus aureus genotyping method based on exotoxin (set) genes.

Fábio Aguiar-Alves1, Fred Medeiros, Octavio Fernandes

  • 1Division of Infectious Diseases and Immunity, School of Public Health, University of California, Berkeley, CA 94720, USA.

Journal of Clinical Microbiology
|August 8, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Influence of inflammatory and reninangiotensin system gene polymorphisms ACE2 rs2285666, IL1A rs1800587, and TNF rs1800629 on COVID-19 severity and the persistence of symptoms in the post-COVID-19 phase: a cross-sectional study.

Einstein (Sao Paulo, Brazil)·2026
Same author

Harnessing interpretable deep learning to predict resistance in <i>Klebsiella pneumoniae</i>.

Frontiers in cellular and infection microbiology·2026
Same author

Antibacterial and Antifungal Properties of <i>Ocotea indecora</i> Essential Oil and Its Nanoemulsion.

Pharmaceuticals (Basel, Switzerland)·2025
Same author

Synthesis, antimicrobial activity, and preliminary mechanistic studies of phenazine sulfonamides.

Bioorganic & medicinal chemistry·2025
Same author

Biocompatible Guanidine-Functionalized Compounds with Biofilm and Membrane Disruptive Activity Against MRSA.

ACS infectious diseases·2025
Same author

Daptomycin Experience in the Pediatric and Neonatal Population: A Systematic Review.

The journal of pediatric pharmacology and therapeutics : JPPT : the official journal of PPAG·2025

A new genotyping method using Staphylococcus aureus superantigen-like protein genes offers a powerful alternative to multilocus sequence typing (MLST). This sequence-based approach provides enhanced discrimination for clinical isolates, aiding epidemiological studies.

Area of Science:

  • Microbiology
  • Genetics
  • Epidemiology

Background:

  • Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) are standard methods for genotyping Staphylococcus aureus.
  • Staphylococcus aureus superantigen-like proteins are encoded by a family of staphylococcal exotoxins.

Purpose of the Study:

  • To develop and evaluate a novel sequence-based genotyping method for Staphylococcus aureus.
  • To compare the discriminatory power of the new method with standard MLST.

Main Methods:

  • PCR amplification of internal fragments of three set genes (set2, set5, set7).
  • Phylogenetic analysis based on single-nucleotide polymorphisms (SNP).
  • Comparison of results with standard seven-gene MLST profiles.

Related Experiment Videos

Main Results:

  • The SNP analysis of set genes differentiated 61 isolates into 22 distinct exotoxin sequence types (ETST).
  • MLST differentiated the same isolates into 19 subgroups.
  • The new method showed equal or greater discriminatory power than MLST, particularly for certain MLST subgroups.

Conclusions:

  • The novel exotoxin-based genotyping method is a valuable alternative to MLST for Staphylococcus aureus.
  • This SNP analysis effectively discriminates isolates based on epidemiological characteristics.
  • The method is suitable for laboratories with sequencing capabilities.