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A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically
Published on: September 9, 2015
Comparison of dalbavancin MIC values determined by Etest (AB BIODISK) and reference dilution methods using
Thomas R Fritsche1, Robert P Rennie, Beth P Goldstein
1JMI Laboratories, 345 Beaver Kreek Centre, Suite A, North Liberty, IA 52317, USA. thomas-fritsche@jmilabs.com
Abstract:
While standardized microdilution testing methodologies and quality control ranges exist for the novel glycolipopeptide dalbavancin, no testing methods have been described that are immediately available for routine use in clinical laboratories. In this study, we found that the dalbavancin Etest (AB BIODISK, Solna, Sweden) procedure demonstrated a high degree of agreement (100% within +/-2 log(2) dilution steps) with the standardized broth microdilution method, validating the use of the Etest as an alternative test for investigational or clinical purposes following regulatory approval.
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