Related Experiment Video
Updated: Aug 6, 2026
Non-Invasive Endotracheal Administration of Lipopolysaccharide to Induce Acute Lung Injury in Rodents
Published on: December 5, 2025
[Effects of dexamethasone on acute lung injury in rats induced by lipopolysacharide]
Qin Lin1, Guang-fa Wang, Xiu-ying Tang
1Department of Respiratory, Peking University First Hospital, Beijing 100034, China.
Objective:
To study the difference between the preventive and therapeutic effects of dexamethasone on acute lung injury models induced by lipopolysacharide (LPS) in different phases.
Methods:
Forty adult male Wistar rats were randomly divided into four groups: (1)control group to receive intraperitoneal NS injection (2 mL/kg). (2)LPS group to receive intraperitoneal LPS injection (5 mg/kg). (3)one-hour group to receive intraperitoneal dexamethasone injection (2 mg/kg) one hour after LPS injection. (4)three-hour group to receive intraperitoneal dexamethasone injection (2 mg/kg) three hours after LPS injection. Then histopathology, arterial blood gases, lung permeability, wet-to-dry weight ratio and immunohistochemistry AQP1 were performed 24 hours later.
Results:
Dexamethasone could improve biological indexes. Lung permeability, wet-to-dry weight ratio and immunohistochemistry AQP1 were (5.73+/-1.37), (4.92+/-0.23), (19.92+/-6.47) in LPS group, (2.4+/-0.51), (4.89+/-0.21), (33.47+/-9.41) in one-hour group and (2.15+/-0.63), (4.57+/-0.14), (40.69+/-9.18) in three-hour group, respectively. Dates in three-hour group were prior to those of one-hour group, and there was slight but no significant difference between the two groups.
Conclusion:
Dexamethasone can improve lung permeability and reduce lung edema. There is no need to be treated with glucocorticoids in advance.
