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Electric field driven jetting: an emerging approach for processing living cells.
Suwan N Jayasinghe1, Peter A M Eagles, Amer N Qureshi
1Materials Department, Queen Mary, University of London, United Kingdom. s.jayasinghe@ucl.ac.uk
Biotechnology Journal
|August 8, 2006
Summary
Electrified jets can process living human Jurkat cells without causing harm. This novel bio-processing method maintains cell integrity and viability, opening new avenues for cellular material manipulation.
Area of Science:
- Biotechnology
- Cellular Engineering
- Electrokinetics
Background:
- Traditional methods for processing cellular materials can cause damage.
- There is a need for non-invasive techniques to handle living cells.
Purpose of the Study:
- To investigate the feasibility of using electrified jets for processing living cellular materials.
- To assess the impact of electrified jetting on human Jurkat cell viability and structural integrity.
Main Methods:
- Living human Jurkat cells in bio-suspensions were processed using electrified jets at electric field strengths of 0.67–2 kV/mm.
- Cells were jetted under sterile conditions, collected, and incubated for 24 and 48 hours.
- Cell survival and structural integrity were evaluated post-processing and incubation.
Main Results:
- Electrified jetting successfully processed Jurkat cells at all tested electric field strengths.
- Cells remained unharmed, retaining their structural integrity and viability after processing and incubation.
- Jetting occurred in an unstable mode, with good correlation between laser spectroscopy and droplet relic measurements.
Conclusions:
- Electrified jets offer a viable method for processing living cellular materials without compromising cell viability.
- This technique demonstrates potential for sterile, non-damaging manipulation of bio-suspensions.
- Further research can explore applications in cellular therapies and tissue engineering.