Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural analysis of mitochondrial pores.

C A Mannella1

  • 1Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.

Experientia
|February 15, 1990
PubMed
Summary

Researchers reveal the structure of a mitochondrial outer membrane channel, modeled as a beta-barrel formed by polypeptides. This provides insights into channel function and potential conformational changes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of divalent metal ions and chelators on the structure of outer mitochondrial membranes from neurospora crassa.

Biophysical journal·2009
Same author

Electron microscopic stains as probes of the surface charge of mitochondrial outer membrane channels.

Biophysical journal·2009
Same author

Structure of the Channels in the Outer Mitochondrial Membrane: Electron Microscopic Studies of the Periodic Arrays Induced by Phospholipase a(2) Treatment of the Neurospora membrane.

Biophysical journal·2009
Same author

Focused ion beam milling of vitreous water: prospects for an alternative to cryo-ultramicrotomy of frozen-hydrated biological samples.

Journal of microscopy·2006
Same author

Topology of the mitochondrial inner membrane: dynamics and bioenergetic implications.

IUBMB life·2002
Same author

Introduction: our changing views of mitochondria.

Journal of bioenergetics and biomembranes·2002

Area of Science:

  • Biochemistry
  • Structural Biology
  • Cell Biology

Background:

  • The mitochondrial outer membrane (MOM) contains protein channels crucial for metabolite transport.
  • Understanding the structure of these channels is key to elucidating their function in cellular bioenergetics and apoptosis.

Purpose of the Study:

  • To present a structural model of the mitochondrial outer membrane channel.
  • To map amino acid distributions and lipid-contact regions within the channel structure.
  • To explore potential conformational changes of the channel.

Main Methods:

  • Analysis of sequence data from channel-forming polypeptides.
  • Electron microscopy of two-dimensional crystals of the channel.
  • Computational modeling based on experimental data.

Main Results:

  • A structural model of the channel as a cylindrical beta-barrel with a 3.8 nm alpha-carbon backbone diameter.
  • Identification of channel-forming polypeptides (30-kDa).
  • Mapping of radial distributions of basic amino acids and lipid-contact regions relative to interchannel bonding sites.

Conclusions:

  • The proposed beta-barrel model provides a framework for understanding channel structure and function.
  • The mapped distributions offer insights into channel assembly and interactions.
  • Further research can explore the dynamic conformational changes of the channel.

Related Experiment Videos