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Updated: Aug 6, 2026
Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates
Published on: April 22, 2019
[Effects of conditioned medium derived from keratinocytes on fibroblasts]
Meng Li1, Linxi Zhang, Qiang Hu
1Department of Plastic Surgery, Xijing Hospital, the Fourth Military Medical University, Xi'an Shaanxi, PR China.
Objective:
To observe the effects of keratinocytes on proliferation and collagen secretion of fibroblasts.
Methods:
The conditioned medium, collected from cultured keratinocytes, was added to the cultured fibroblasts as the tested groups (12.5%, 25% and 50% groups) and DMEM as control group. The MTT, hydroxyproline colorimetric method and flow cytometer were employed to measure the fibroblast proliferation, the collagen secretion and the change of the cell cycle.
Results:
In fibroblast proliferation, the absorbency(A) value of tested groups was significantly different from that of the control group (P<0.01). A value increased as increasing concentration, there was statistically significant difference between the concentrations of 25%, 50% and the concentration of 12.5% (P<0.01), but no statistically significant difference between the concentrations of 25% and 50% (P>0.01). In collagen secretion, there was no statistically significant difference between the tested groups and the control group(P>0. 01), and between the tested groups(P>0.01). In cell cycle, 50% of conditioned medium could make the fibroblast pass the limit of G1/S and S/G2 period, the cell rates of S,G2-M period increased.
Conclusion:
The conditioned medium from keratinocytes can increase fibroblasts proliferation, have little effect on general collagen secretion.
