Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Modulation of vitronectin receptor binding by membrane lipid composition.

G Conforti1, A Zanetti, I Pasquali-Ronchetti

  • 1Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy.

The Journal of Biological Chemistry
|March 15, 1990
PubMed
Summary

The vitronectin receptor

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Search for Physics beyond the Standard Model in Events with Overlapping Photons and Jets.

Physical review letters·2020
Same author

Measurements of triple-differential cross sections for inclusive isolated-photon+jet events in <math></math> collisions at <math> </math>.

The European physical journal. C, Particles and fields·2019
Same author

Measurement of the average very forward energy as a function of the track multiplicity at central pseudorapidities in proton-proton collisions at <math> </math>.

The European physical journal. C, Particles and fields·2019
Same author

Search for Low-Mass Quark-Antiquark Resonances Produced in Association with a Photon at sqrt[s]=13  TeV.

Physical review letters·2019
Same author

Search for new physics in top quark production in dilepton final states in proton-proton collisions at <math> </math>.

The European physical journal. C, Particles and fields·2019
Same author

Azimuthal separation in nearly back-to-back jet topologies in inclusive 2- and 3-jet events in <math></math> collisions at <math> </math>.

The European physical journal. C, Particles and fields·2019

Area of Science:

  • Cell biology
  • Biochemistry
  • Membrane protein research

Background:

  • The vitronectin receptor, an integrin protein, has a well-defined structure but poorly understood binding properties.
  • Integrin-mediated cell adhesion plays a critical role in various physiological and pathological processes.

Purpose of the Study:

  • To investigate how membrane lipid composition affects the binding affinity and specificity of the vitronectin receptor.
  • To explore the relationship between receptor conformation and its interaction with matrix proteins.

Main Methods:

  • Reconstituting purified vitronectin receptor into liposomes with varying lipid compositions (phosphatidylcholine, PC; PC+phosphatidylethanolamine, PE; PC+PE+phosphatidylserine, PS+phosphatidylinositol, PI+cholesterol, chol).
  • Assessing liposome binding capacity to vitronectin (Vn), von Willebrand factor (vWF), and fibronectin (Fn) using electron microscopy and antibody blocking assays.
  • Analyzing conformational changes using intrinsic emission fluorescence spectroscopy.

Main Results:

  • Vitronectin receptor binding specificity is significantly influenced by the surrounding membrane lipids.
  • Receptors in liposomes with more complex lipid compositions (PC+PE and PC+PE+PS+PI+chol) bound to Vn, vWF, and Fn, unlike those in simple PC liposomes.
  • Increased lipid complexity correlated with higher binding capacity and induced conformational changes in the receptor.

Conclusions:

  • The vitronectin receptor exhibits promiscuous binding to Vn, vWF, and Fn.
  • Membrane lipid composition modulates vitronectin receptor binding affinity and specificity, likely through conformational alterations.

Related Experiment Videos