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A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells
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Increased association of dynamin II with myosin II in ras transformed NIH3T3 cells

Soon-Jeong Jeong1, Su-Gwan Kim, Jiyun Yoo

  • 1Department of Oral Histology, College of Dentistry, Chosun University, Gwangju 501-759, South Korea.

Acta Biochimica Et Biophysica Sinica
|August 9, 2006
PubMed

Insights

Dynamin II and myosin II interact and influence cell migration. This interaction is modulated by platelet-derived growth factor and involves the Ras signaling pathway, suggesting dynamin II

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Dynamin is involved in vesicle formation via endocytic and secretory pathways.
  • Dynamin also influences cell membrane shape during cell migration, interacting with cytoskeleton proteins.
  • Myosin II is crucial for maintaining cell morphology and driving cellular movement.

Purpose of the Study:

  • To investigate the potential relationship between dynamin II and myosin II.
  • To explore the role of dynamin II and myosin II in cell migration signaling.

Main Methods:

  • Reciprocal immunoprecipitation to detect protein interactions.
  • Confocal microscopy to visualize protein localization.
  • Co-expression studies in NIH3T3 cells (normal and Ras-transformed).
  • Treatment with platelet-derived growth factor (PDGF).

Main Results:

  • Dynamin II and myosin II were found to interact.
  • Dynamin II expression was elevated when co-expressed with myosin II in Ras-transformed cells.
  • Platelet-derived growth factor treatment altered dynamin II and myosin II interaction and localization, inducing lamellipodia and cell migration.
  • Grb2 adaptor protein associated with dynamin II and myosin II gene expression in Ras-transformed cells.

Conclusions:

  • Dynamin II and myosin II interact and are involved in PDGF-induced cell migration.
  • Dynamin II may act as an intermediate messenger in the Ras signal transduction pathway.
  • The dynamin II-myosin II complex influences membrane ruffling and cell migration.

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