Related Experiment Video
Updated: Aug 6, 2026
Detection and Isolation of Cancer in Prostate Biopsies Using Stimulated Raman Histology and Artificial Intelligence
Published on: June 10, 2025
Screening of genetic and expression alterations of SRC1 gene in prostate cancer
Hanna E Mäki1, Kati K Waltering, Mika J Wallén
1Cancer Genetics, Institute of Medical Technology, University of Tampere and Tampere University Hospital, Tampere, Finland.
Background:
Genetic alterations of the SRC1 gene have not been thoroughly studied in prostate cancer.
Materials And Methods:
Five prostate cancer cell lines and 32 xenografts were screened for mutations and gene copy number alterations. Subsequently, frequencies of detected sequence variations were further analyzed in 44 clinical prostate cancers, 6 benign prostate hyperplasias, and 48 normal controls. Finally, the protein expression of SRC1 in 254 clinical prostate tumors was investigated.
Results:
Three non-recurrent sequence variations, and one single nucleotide polymorphism in the coding region of SRC1, as well as one case of SRC1 gene amplification were found. The protein expression of SRC1 was higher in androgen ablation resistant than untreated prostate carcinomas, but the difference was not statistically significant (P = 0.0796).
Conclusions:
Genetic alterations of SRC1 are rare in prostate cancer. The nuclear protein accumulation of SRC1 seems to be mildly increased in androgen ablation resistant prostate cancers. .
Insights
Genetic alterations of the SRC1 gene are uncommon in prostate cancer. While SRC1 protein levels may slightly increase in resistant tumors, further research is needed.
Area of Science:
- Oncology
- Genetics
- Molecular Biology
Background:
- The role of SRC1 gene alterations in prostate cancer remains largely unexplored.
- Understanding SRC1's genetic landscape is crucial for identifying potential therapeutic targets.
Purpose of the Study:
- To investigate the frequency and types of genetic alterations in the SRC1 gene in prostate cancer.
- To examine the protein expression levels of SRC1 in relation to prostate cancer progression and treatment resistance.
Main Methods:
- Screening of prostate cancer cell lines and xenografts for SRC1 mutations and copy number variations.
- Analysis of SRC1 sequence variations in clinical prostate cancer samples, benign prostate hyperplasia, and normal controls.
- Quantification of SRC1 protein expression in a cohort of prostate tumors.
Main Results:
- Rare genetic alterations, including sequence variations and one gene amplification, were identified in SRC1.
- SRC1 gene amplification was detected in one case.
- A non-significant trend towards higher SRC1 protein expression was observed in androgen ablation-resistant prostate cancers compared to untreated ones.
Conclusions:
- Genetic alterations affecting the SRC1 gene are infrequent in prostate cancer.
- A mild increase in nuclear SRC1 protein accumulation may occur in advanced, treatment-resistant prostate cancers.
