Recent developments in the HPLC enantiomeric separation using chiral selectors identified by a combinatorial strategy

Area of Science:

• Analytical Chemistry
• Chromatography

Background:

• Chiral stationary phases (CSPs) are crucial for enantiomer separation in chromatography.
• Two types of CSPs exist: conventional with unpredictable selectivity and those with predictable elution orders.
• Developing CSPs with predictable selectivity is an active area of research.

Purpose of the Study:

• To review recent advances in High-Performance Liquid Chromatography (HPLC) applications.
• To focus on chiral selectors identified through combinatorial approaches for CSP development.
• To discuss methodologies for creating chiral selectors with predictable elution orders.

Main Methods:

• Exploration of combinatorial approaches for chiral selector development.
• Review of molecular imprinting technology and antibody production as alternative methods.
• Focus on two categories of combinatorial procedures: small libraries of low-molecular weight selectors and large libraries of oligonucleotide aptamers (DNA and RNA).

Main Results:

• Recent advances in HPLC applications of chiral selectors derived from combinatorial methods are presented.
• The review details the use of both small molecule libraries and aptamers in CSP development.
• The effectiveness of these combinatorial approaches in achieving predictable enantioseparation is discussed.

Conclusions:

• Combinatorial approaches offer powerful strategies for designing chiral selectors with predictable selectivity.
• Oligonucleotide aptamers represent a promising avenue for developing highly specific CSPs.
• These advancements enhance the capabilities of HPLC for chiral separations.