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Updated: Aug 6, 2026

Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1
Published on: August 24, 2015
[Expression of multi-copy and immuno-reactivity of recombinant type-specific epitope of herpes simplex virus type 1]
Xiao-wei Ji1, Xu-hu Mao, Quan-ming Zou
1Department of Clinical Microbiology and Immunology, the Third Military Medical University and Chongqing Research Center of Biopharmaceutical Engineering Technology, Chongqing 400038, China.
Objective:
To express a multi-copy specific epitope recombinant protein of herpes simplex virus type 1 (HSV-1) with immuno-reactivity.
Methods:
Multi-copy genes with a specific epitope of HSV-1-glycoprotein G 112-127 were constructed by DNA recombination and cloned in E. coli JM109 pGEM-5Zf. The positive recombinants were determined by SDS-PAGE and Western blotting.
Results:
The recombinants with 4, 8, 16 and 32 copies of gG 112-127 were obtained. The 8-copy recombinant was expressed by 17.5%, mainly as inclusion body. And it reacted with antiserum HSV-1, but not with antiserum HSV-2.
Conclusion:
The HSV-1-gG112-127 recombinant could be used to distinguish HSV-1 and HSV-2 in ELISA.
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