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Updated: Jan 9, 2026
Author Spotlight: A Live Cell Imaging Technique to Study Calcium Signaling and Acrosome Exocytosis in Mouse Sperm
Published on: October 13, 2023
[Effects of flunarizine on T-type calcium channels in mouse spermatogenic cells]
Chang-song Wang1, Xiao-hua Gao, Hong Cheng
1Institute of Applied Toxicology of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Objective:
To investigate the effects of Flunarizine (Flu) on T-type calcium currents (ICaT) in spermatogenic cells.
Methods:
Ca2+ currents were obtained in acutely dissociated mouse spermatogenic cells using whole-cell patch clamp technique and the effects of Flu on ICaT were observed.
Results:
Flu of 0.1, 1, 10, 100 micromol/L inhibited ICaT in mouse spermatogenic cells significantly with the K50 value of 0.289 micromol/L (P < 0.05). With the holding potential at -90 mV and stimulating potential at -30 mV, the inhibition rates of Flu were (23.34 +/- 2.76)%, (46.04 +/- 3.52)%, (62.52 +/- 3.70)% and (73.52 +/- 3.12)%, respectively.
Conclusion:
Flu has significant inhibitory effects on ICaT in mouse spermatogenic cells, with concentration dependence. Ca(v)3.2 is the main contributor to T-type Ca2+ currents in spermatogenic cells.
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