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Updated: Jul 14, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array
Published on: November 15, 2017
A binary DNA probe for highly specific nucleic Acid recognition
1Division of Experimental Therapeutics, Department of Medicine, Columbia University, Box 84, 630W 168th Street, New York, New York 10032, USA. dk2111@clumbia.edu
Abstract:
A new concept for nucleic acid probe design is reported. The extremely high selectivity of the probe is predetermined by cooperative hybridization of the two relatively short (10 nucleotide) DNA hairpin fragments to the analyte. A binary DNA probe fluorescently reports the presence of 0.5% of the analyte in excess amount of a single base substituted oligodeoxyribonucleotide and distinguishes single nucleotide substitutions at any position of a 20-mer oligonucleotide at room temperature.
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