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Updated: Aug 6, 2026

Fluorescence Imaging with One-nanometer Accuracy (FIONA)
Published on: September 26, 2014
Nanoscale resolution in GFP-based microscopy
Katrin I Willig1, Robert R Kellner, Rebecca Medda
1Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany.
Abstract:
We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The approximately 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.
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