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Related Concept Videos

Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Anaphase A and B01:39

Anaphase A and B

Microtubules form through the end-to-end polymerization of tubulin heterodimers. Kinetochore microtubules originate from the spindle poles, and their plus-ends connect with the kinetochores on sister-chromatids. Ndc80 protein complexes, present on the kinetochore, form low-affinity links with the plus end of these kinetochore microtubules.
Plus-end depolymerization releases tubulin heterodimers from the terminal region of the microtubule. As tubulin subunits are lost, the Ndc80 complexes detach...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Anaphase A and B01:39

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Microtubules form through the end-to-end polymerization of tubulin heterodimers. Kinetochore microtubules originate from the spindle poles, and their plus-ends connect with the kinetochores on sister-chromatids. Ndc80 protein complexes, present on the kinetochore, form low-affinity links with the plus end of these kinetochore microtubules.
Plus-end depolymerization releases tubulin heterodimers from the terminal region of the microtubule. As tubulin subunits are lost, the Ndc80 complexes detach...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

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Updated: May 11, 2026

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
10:54

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

Published on: September 17, 2012

The anaphase promoting complex/cyclosome: a machine designed to destroy.

Jan-Michael Peters1

  • 1Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria. peters@imp.univie.ac.at

Nature Reviews. Molecular Cell Biology
|August 10, 2006
PubMed
Summary
This summary is machine-generated.

The anaphase promoting complex/cyclosome (APC/C) is a complex ubiquitin ligase crucial for the cell cycle. Recent studies reveal novel mechanisms controlling its activity and substrate recognition.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • The anaphase promoting complex/cyclosome (APC/C) is a large, multi-subunit ubiquitin ligase essential for eukaryotic cell cycle progression.
  • It catalyzes ubiquitylation reactions, playing critical roles both within and outside of cell cycle control.
  • Understanding APC/C function is vital due to its complexity and fundamental biological importance.

Purpose of the Study:

  • To elucidate the intricate mechanisms regulating the activity of the anaphase promoting complex/cyclosome (APC/C).
  • To gain insights into how the APC/C, a complex molecular machine, recognizes and targets its diverse substrates.
  • To explore the multifaceted roles of APC/C beyond its canonical cell cycle functions.

Main Methods:

  • Structural biology techniques to visualize the APC/C complex.
  • Biochemical assays to study ubiquitylation activity.
  • Genetic approaches to investigate regulatory mechanisms and substrate interactions.

Main Results:

  • Identification of novel regulatory pathways controlling APC/C assembly and function.
  • Characterization of specific substrate recognition motifs and adaptor proteins.
  • Demonstration of APC/C's involvement in diverse cellular processes, extending beyond cell cycle regulation.

Conclusions:

  • The APC/C is a highly regulated enzyme with complex substrate recognition mechanisms.
  • Recent discoveries highlight a broader functional spectrum for APC/C in cellular processes.
  • Further research into APC/C regulation and function promises new therapeutic targets.