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Related Experiment Videos

Idiotype-specific T helper clones recognize a variable H chain determinant.

Y Saeki1, J J Chen, L F Shi

  • 1IDEC Pharmaceuticals Corporation, La Jolla, CA 92037.

Journal of Immunology (Baltimore, Md. : 1950)
|March 1, 1990
PubMed
Summary
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Regulatory T cells recognize specific antibody fragments, particularly the H chain, as their target idiotype. This recognition mechanism may offer biological advantages in antigen presentation.

Area of Science:

  • Immunology
  • Molecular Biology
  • T cell biology

Background:

  • Previously, a T helper (Th) cell clone specific for a regulatory idiotype on the 3A4 anti-idiotype monoclonal antibody (mAb) was described.
  • The 3A4 mAb mimics a tumor-associated antigen found on murine L1210/GZL cells.

Purpose of the Study:

  • To determine the precise molecular target recognized by the Th clone on the stimulating anti-idiotype antibody.
  • To investigate the role of isolated antibody chains and their presentation in T cell activation.

Main Methods:

  • T helper cell proliferation assays using isolated antibody chains (H and L) and fragments (Fab) of the 3A4 mAb.
  • Antigen-presenting cell (APC) presentation studies involving chloroquine treatment to assess antigen processing resistance.

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Main Results:

  • The Th clone demonstrated specific proliferation in response to the H chain of 3A4, but not the L chain.
  • Isolated 3A4 H chain stimulated the Th clone more effectively than intact 3A4 or Fab fragments.
  • APC presentation of the H chain was more resistant to chloroquine treatment compared to intact 3A4.

Conclusions:

  • Regulatory T cells recognize target idiotopes as linear sequence determinants on isolated immunoglobulin chains.
  • This recognition and presentation mechanism of isolated H chains may confer biological advantages in antigen presentation processes.