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A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
04:23

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Published on: April 28, 2019

Cellular immune activation in children with acute dengue virus infections is modulated by apoptosis

Khin S Myint1, Timothy P Endy, Duangrat Mongkolsirichaikul

  • 1Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.

The Journal of Infectious Diseases
|August 10, 2006
PubMed

Insights

Apoptosis, programmed cell death, is elevated in children with severe dengue hemorrhagic fever (DHF) compared to dengue fever (DF). This finding highlights apoptosis

Area of Science:

  • Immunology
  • Virology
  • Cell Biology

Background:

  • Apoptosis regulates immune responses during systemic viral infections.
  • CD95 is a key mediator of apoptosis.

Purpose of the Study:

  • To investigate peripheral-blood mononuclear cell (PBMC) apoptosis and soluble CD95 levels in children with dengue virus (DV) infections.
  • To correlate apoptosis levels with dengue disease severity.

Main Methods:

  • Prospective study of children with DV infections.
  • Sequential sampling for PBMC apoptosis and plasma soluble CD95 levels.
  • Analysis of apoptotic cell populations, including CD8(+) T lymphocytes.

Main Results:

  • Higher PBMC apoptosis levels were observed during defervescence in children with dengue hemorrhagic fever (DHF) compared to dengue fever (DF) and other febrile illnesses.
  • CD8(+) T lymphocytes constituted a significant portion of apoptotic PBMCs in DHF and DF.
  • Maximum plasma soluble CD95 levels were elevated in children with DHF compared to DF.
  • PBMC apoptosis levels correlated positively with dengue disease severity.

Conclusions:

  • Apoptosis plays a role in modulating innate and adaptive immune responses during DV infection.
  • Elevated apoptosis and soluble CD95 are associated with severe dengue illness.
  • Apoptosis may be involved in the pathogenesis of viral hemorrhagic fevers.

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