Synthesis of poly d(G-C) oligonucleotides
1Illumina, Inc., 9885 Towne Centre Dr., San Diego, CA 92130, USA.
Nucleosides, Nucleotides & Nucleic Acids
|August 11, 2006
Abstract:
Model sequences for evaluation of the GC dimer sequence repetition on synthesis success were prepared and analyzed by HPLC. Contiguous d(G-C) or d(C-G) sequences have a deleterious effect on DNA oligonucleotide synthesis. The critical number seems to be about 6 GCs in a row. If the GCs are separated by other nucleotides, the effect is not as severe.
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