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Related Experiment Videos

Positive stem cell selection--basic science.

C I Civin1, L C Strauss, M J Fackler

  • 1Johns Hopkins Oncology Center, Baltimore, Maryland 21205.

Progress in Clinical and Biological Research
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

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Positive selection using CD34 monoclonal antibodies can isolate hematopoietic stem cells for autologous bone marrow transplantation. This method shows promise for engraftment and broad clinical applicability in bone marrow therapies.

Area of Science:

  • Immunology
  • Hematology
  • Cell Biology

Background:

  • Autologous bone marrow transplantation often uses immunologic purging of malignant cells.
  • Positive selection of lymphohematopoietic stem cells is an alternative strategy.
  • CD34 is a cell surface glycoprotein marker for hematopoietic progenitor cells.

Purpose of the Study:

  • To evaluate the utility of CD34 monoclonal antibodies for positive selection in autologous bone marrow transplantation.
  • To determine if CD34+ cells include hematopoietic stem cells capable of engraftment.

Main Methods:

  • Characterization of CD34 antigen expression on hematopoietic cells and malignancies.
  • Large-scale isolation of CD34+ cells using immunomagnetic microspheres or columns.
  • Transplantation of CD34+ cells into myeloblated recipients (non-human primates and human patients).

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Main Results:

  • CD34 is expressed on precursors of all major hematopoietic lineages.
  • Mature blood cells and most hematopoietic malignancies do not express CD34.
  • Isolated CD34+ cells demonstrated short-term hematopoietic engraftment in recipients.

Conclusions:

  • CD34 monoclonal antibodies are suitable for positive selection of hematopoietic stem cells.
  • CD34+ cell transplantation shows potential for broad application in bone marrow transplantation.
  • This approach offers a promising alternative to negative selection for purging malignant cells.