Characterisation of dendritic cell subsets in lung cancer micro-environments
A Bergeron1, F El-Hage, M Kambouchner
1Université Paris 13, UPRES EA-3406, Assistance Publique-Hôpitaux de Paris, Hôpital Avicenne, Laboratoire d'hématologie biologique, Bobigny, France.
The European Respiratory Journal
|August 11, 2006
Summary
Dendritic cells (DCs) infiltrate lung tumors, with specific subsets correlating to chemokine levels. These immune cells exhibit an immature phenotype due to the tumor
Area of Science:
- Immunology
- Oncology
- Cell Biology
Background:
- Lung cancer micro-environments contain various immune cells.
- Dendritic cells (DCs) play a crucial role in anti-tumor immunity.
- Understanding DC subsets and their interaction with tumors is vital for immunotherapy.
Purpose of the Study:
- To investigate the correlation between lung tumor micro-environment mediators and infiltrating dendritic cell (DC) subsets.
- To determine the phenotype of DCs within lung carcinomas.
- To evaluate the expression of chemokines and cytokines influencing DC recruitment and maturation.
Main Methods:
- Immunohistochemistry with novel antibodies on surgical biopsies from 12 lung carcinoma patients.
- Real-time PCR for mRNA analysis of cytokines.
- Evaluation of chemokine mRNA and protein expression.
Main Results:
- Myeloid dendritic cell (DC) subsets, including Langerhans cells and CD1a+/Langerin+ cells, were found within tumors.
- The number of certain DC subsets correlated with CC chemokine ligand 20 (CCL20) expression.
- Tumors expressed inhibitory cytokines (IL-10, TGF-β, VEGF) and lacked IL-12, associated with an immature DC phenotype.
Conclusions:
- Various dendritic cell (DC) subsets infiltrate lung carcinomas.
- DCs within the tumor micro-environment display an immature phenotype.
- This immaturity is likely driven by the local expression of inhibitory cytokines.
