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Electron microscopic observations of 20 human osteosarcomas
Clinical Orthopaedics and Related Research
|September 1, 1975
Summary
Electron microscopy revealed common cellular abnormalities in osteosarcoma bone and lung metastases, including irregular nuclei and abundant endoplasmic reticulum. These fine structure changes suggest increased metabolic activity but lack definitive diagnostic features.
Area of Science:
- Oncology
- Cell Biology
- Electron Microscopy
Background:
- Osteosarcoma is a primary bone cancer with diverse histological features.
- Understanding the ultrastructural characteristics of osteosarcoma is crucial for potential diagnostic markers.
Purpose of the Study:
- To investigate the fine structure of osteosarcoma cells using electron microscopy.
- To identify any common ultrastructural abnormalities and their potential implications.
Main Methods:
- Electron microscopy was performed on twenty primary osteosarcomas and two lung metastases.
- Cell cultures derived from osteosarcomas were also examined.
Main Results:
- Common abnormalities in cell configuration and fine structure were observed, including spindle-shaped cells, enlarged irregular nuclei, and prominent nucleoli.
- Abundant, disorderly rough endoplasmic reticulum and numerous mitochondria were noted.
- Disorganized collagenous matrix was present; no definitive virus particles were seen, though some structures resembled unenveloped nucleocapsids.
Conclusions:
- Observed fine structure abnormalities may indicate increased metabolic rate and abnormal protein/enzyme synthesis.
- No definitive diagnostic features were identified through electron microscopy.
- Cell cultures exhibited similar fine structure to fresh tissue, with no virus-like particles observed.