Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene expression within a dynamic nuclear landscape.

Yaron Shav-Tal1, Xavier Darzacq, Robert H Singer

  • 1The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, Israel. shavtaly@mail.biu.ac.il

The EMBO Journal
|August 11, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Elevated MyoD1 levels expand genome-wide binding and the repertoire of regulated genes.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

An integrated RNA-centric imaging and omics approach reveals distinct properties and composition of neuronal RNA granules.

bioRxiv : the preprint server for biology·2026
Same author

RNA-binding proteins in the mouse lens: Functional classifications, expression profiling, and interaction studies of Carhsp1 with crystallin mRNAs.

Developmental biology·2026
Same author

A series of spontaneously blinking dyes for super-resolution microscopy.

Nature methods·2026
Same author

Unstructured transcription factor interactions enable emergent specificity.

Science (New York, N.Y.)·2026
Same author

A platform for analyzing translational control by RBPs at single-mRNA resolution in cells.

Biophysics and physicobiology·2026
Same journal

Chromosome condensation mechanically primes the nucleus for mitosis.

The EMBO journal·2026
Same journal

NDR kinase SAX-1 controls dendrite branch-specific elimination during neuronal remodeling in C. elegans.

The EMBO journal·2026
Same journal

Assembly of the catalytic module and the rotor of human ATP synthase.

The EMBO journal·2026
Same journal

Substrate-induced assembly and functional mechanism of the membrane protein insertase SecYEG-YidC.

The EMBO journal·2026
Same journal

Conformational changes of the baseplate regulating tail contraction of Staphylococcus phage 812.

The EMBO journal·2026
Same journal

Cellular assembly and functional resilience of the mammalian RNA exosome.

The EMBO journal·2026
See all related articles

Single-cell imaging reveals how molecular processes in live cells vary. Time-resolved analysis of individual cells, not population averages, is crucial for understanding cell biology and gene expression dynamics.

Area of Science:

  • Cell Biology
  • Molecular Imaging
  • Biophysics

Background:

  • Molecular imaging in living systems offers unprecedented temporal resolution for studying macromolecular assemblies.
  • Emerging imaging technologies are reshaping our understanding of cell biology by revealing novel concepts.
  • The inherent stochasticity of cellular processes suggests population-level analysis may obscure critical single-cell behaviors.

Purpose of the Study:

  • To review technological advancements in molecular imaging for live-cell analysis.
  • To discuss the importance of time-resolved single-cell experiments over population studies.
  • To explore how noninvasive single-cell approaches reveal nuclear organization and molecular processes.

Main Methods:

  • Utilizing advanced molecular imaging techniques for time-resolved observation of cellular events.

Related Experiment Videos

  • Employing noninvasive single-cell approaches to investigate nuclear structure and molecular dynamics.
  • Reviewing technological developments in imaging and their application to stochastic gene expression.

    • Main Results:

    • Single-cell imaging demonstrates significant stochastic variation among cells, challenging traditional population-based analyses.
    • Time-resolved single-cell experiments provide insights into cause-and-effect relationships at the molecular level.
    • Noninvasive approaches enable high-resolution analysis of molecular processes within the nucleus of live cells.

    Conclusions:

    • Analysis of individual cells over time is essential for a comprehensive understanding of cell biology.
    • Molecular imaging provides the resolution needed to study complex molecular processes in live cells.
    • Future efforts focus on integrating these single-cell insights into physiological studies of living organisms.