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Published on: November 29, 2019
Cystatin C in cerebrospinal fluid and multiple sclerosis
Sara F Hansson1, Anja Hviid Simonsen, Henrik Zetterberg
1Department of Clinical Neuroscience, University of Göteborg, Sahlgrenska University Hospital/Mölndal, Mölndal, Sweden.
Annals of Neurology
|August 11, 2006
Summary
A truncated 12.5 kDa cystatin C (CysC) isoform, initially identified as a multiple sclerosis (MS) biomarker, is actually a storage artifact. This finding indicates it is not a reliable diagnostic marker for MS.
Area of Science:
- Biochemistry
- Neuroimmunology
Background:
- Cerebrospinal fluid (CSF) analysis is crucial for diagnosing neurological disorders.
- Biomarkers for multiple sclerosis (MS) are actively sought to improve diagnostic accuracy.
Purpose of the Study:
- To investigate the diagnostic utility of a 12.5 kDa truncated cystatin C (CysC) isoform in multiple sclerosis (MS).
- To determine the origin and significance of the 12.5 kDa CysC isoform in cerebrospinal fluid.
Main Methods:
- Surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) analysis was performed on CSF samples.
- CSF samples were collected from 43 individuals with MS and 46 healthy controls.
Main Results:
- No significant difference in full-length CysC (13.4 kDa) concentration was observed between MS patients and controls.
- The 12.5 kDa CysC isoform was identified as a degradation product of full-length CysC.
- This degradation occurred due to N-terminal cleavage during sample storage at -20°C.
Conclusions:
- The 12.5 kDa CysC isoform is an artifact generated during sample storage.
- This truncated CysC isoform is not a valid biomarker for diagnosing multiple sclerosis.
- Re-evaluation of sample handling and analysis is necessary for accurate biomarker discovery.
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