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Leishman-Giemsa cocktail: an effective Romanowsky stain for air-dried cytologic smears
Rajendra S Garbyal1, Neeta Agarwal, Prachi Kumar
1Department of Pathology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India. rajgarbyal@yahoo.com
Objective:
To develop an economical, quick, readily available and simple alternative to May-Grünwald-Giemsa (MGG) stain and so to explore the combination of Leishman and Giemsa stain (LG cocktail).
Study Design:
One wet-fixed and 2 air-dried smears were prepared from 720 cases during the period January 2003-November 2004. The LG cocktail and MGG stain were used on the air-dried smears and Papanicolaou stain in wet-fixed smears. Diagnoses of the cases were made using the LG cocktail-stained smears, and then its diagnostic efficacy was cross-checked with the MGG- and Papanicolaou-stained smears by the same cytopathologist. A comparative study of the LG cocktail and MGG-stained smears was done.
Results:
The results achieved with the LG cocktail-stained smears were comparable to or even better than those with the MGG-stained smears, with excellent diagnostic efficacy.
Conclusion:
As compared to MGG stain, the 1-step LG cocktail is cheaper, easier to standardize and quicker.
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